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KMID : 1140120050100040242
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Cancer Prevention Research
2005 Volume.10 No. 4 p.242 ~ p.250
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Protective Effects of Liliaceae Root Extracts on Amyloid β-protein-induced Death in Neuronal Cells
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Kim Eun-Sook
Choi Soo-Jin
Lee Chul-Won
Park Ki-Tae
Moon Ja-Young
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Abstract
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The amyloid β-protein (Aβ) is the principal component of the senile plaques characteristic of Alzheimer’s disease (AD) and elicits a toxic effect on neurons in vitro and in vivo. Many environmental factors including antioxidants and proteoglycans modify Aβ toxicity. In this study, we have investigated the effects of methanol-extracts of Liliaceae root on Aβ-induced oxidative cell death in cultured rat pheochromocytoma (PC12) cells. For this study, we used three different Aβ fragments (Aβ25-35, Aβ1-42 Aβ1-43) for the induction of cytotoxicities to PC12 cells. We previously measured cytotoxicities of the extracts of Liliaceae to both human neuronal SK-N-MC and PC12 cells. Fifteen out of the 19 Liliaceae extracts at 100μg/ml showed no cytotoxic effects to both SK-N-MC and PC12 cells. Three different Aβ fragments (Aβ25-35, Aβ1-42 Aβ1-43) exhibited cytotoxicities to both SK-N-MC and PC12 cells, each possessing 1, 5, and 5μg/ml IC50 values, respectively. Among the Liliaceae roots, methanol-extracts of Polygonatum falcatum, Ophiopogon japonicus, Liriope platyphylla, and Hemerocallis fulva strongly attenuated cytotoxicities induced by the three Aβ fragments. Especially methanol-extracts of Ophiopogon japonicus was the most effective in protecting PC12 cells from the Aβ25-35-induced oxidative cell death. These results suggest that extracts of Liliaceae root may contain the protective component (s) against Aβ-induced cell death in PC12 cells.
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KEYWORD
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Amyloid β-protein, Liliaceae, Oxidative stress, PC12, Neuronal SK-N-MC
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